HPLC–CLND for the Analysis of Underivatized Amino Acids

Performance of 2-Amino Tetraphenyl Porphyrin as Stationary Phase in RP-HPLC of Amino Acids

The search for new stationary phases has been one of the predominant concerns in high performance liquid chromatography (HPLC) in order to achieve better resolutions, longer column lives, and reduce the time of analysis. A chromatographic packing for separation of underivatized amino acids (AAs) were prepared by dynamically coating 2-amino tetraphenyl prophyrin (atpp) on a C-18 reversed-pha...

HPLC – CLND for the Analysis of Underivatized

Analysis of Plasma Amino Acids Using RP-HPLC and Pre-Column Derivatization with OPA/3-MPA

ABSTRACT        Background and Objective: Measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. The aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (RP-HPLC) with pre-column derivatization by o-phthalaldehyde (OPA)...

Validated quantitation of underivatized amino acids in human blood samples by volatile ion-pair reversed-phase liquid chromatography coupled to isotope dilution tandem mass spectrometry.

Quantitation of amino acids in complex matrixes without derivatization is advantageous; however, difficulties exist in both the separation and the detection of those compounds. A validated method that is based on the use of volatile ion-pair liquid chromatography coupled to stable isotope dilution tandem mass spectrometry has been developed for the simple and accurate quantitation of underivati...

Optimization of the Analytical Conditions for Amino Acid Profiling Using a Sheathless Ce/esi-ms

We report a robust strategy for amino acid profiling method based on capillary electrophoresiselectrospray ionization-mass spectrometry (CE/ESI-MS) that offers a convenient platform for the direct analysis of underivatized amino acids from dried blood spot without any chemical derivatization. Sample preparation steps were simplified without derivatization. The analytical conditions with respect...